At a Glance
Why Get Tested?
To determine whether a person has sickle cell trait or disease by measuring the presence and amount of hemoglobin S
When to Get Tested?
A blood sample drawn from a vein in your arm or obtained from the heel or finger of an infant
Test Preparation Needed?
None; however, if this test is used for diagnosis, the sample should not be drawn after a recent blood transfusion.
The Test Sample
What is being tested?
Sickle cell tests are used to detect, diagnose, and confirm sickle cell anemia (also called sickle cell disease) and to identify those who may have sickle cell trait. Sickle cell anemia is an inherited disorder that leads to the production of an abnormal hemoglobin called hemoglobin S (Hb S or Hgb S). Hemoglobin is a protein found in red blood cells (RBCs) that binds to oxygen in the lungs and carries it to tissues throughout the body.
Typically, hemoglobin A (Hb A) makes up most of the hemoglobin found in normal RBCs in adults, with small amounts of hemoglobin A2 and hemoglobin F. Before babies are born, they normally produce large amounts of hemoglobin F (Hb F), which is then slowly replaced by Hb A after birth. Mutations in the genes that code for the production of hemoglobin can lead to abnormal types of hemoglobin. Common mutations include beta thalassemia and those associated with hemoglobin variants such as Hb S, hemoglobin C (Hb C). With a normal hemoglobin gene copy from one parent and a Hb S gene copy from the other parent (heterozygous), a person is said to have sickle cell trait and be a sickle cell carrier. When a person has two Hb S gene copies (one from each parent; homozygous), then he has sickle cell anemia (disease). If he has one Hb S gene and one other abnormal gene, such as Hb C gene, then he will experience some of the same symptoms associated with sickle cell disease.
Hb S can form crystals that change the shape of the RBC from a round disc to a characteristic sickle shape. This altered shape limits the RBC's ability to flow smoothly throughout the blood vessels in the body, limits the hemoglobin's ability to transport oxygen to tissues, and decreases the RBCs' lifespan from 120 days to about 10-20 days. A person with sickle cell disease (homozygous for Hb S) can become severely anemic because the body cannot produce RBCs as fast as they are destroyed. The affected person can suffer painful episodes and a variety of complications when sickled cells become lodged in and obstruct small blood vessels.
Sickle cell tests are done to determine whether someone is producing hemoglobin S, thus carrying a sickle gene. They are ordered routinely as part of newborn screening programs and are mandated by every state in the US and the District of Columbia. If results of a newborn screen are abnormal, then one or more sickle cell tests may be ordered to confirm abnormal findings. Sickle cell tests may also be ordered along with or following an abnormal CBC and blood smear, with normal iron studies to help evaluate a patient who has an unexplained hemolytic anemia or demonstrate symptoms that suggest the presence of sickle cell anemia.
How is the sample collected for testing?
A blood sample is obtained by inserting a needle into a vein or, in infants, by pricking a heel or finger.
NOTE: If undergoing medical tests makes you or someone you care for anxious, embarrassed, or even difficult to manage, you might consider reading one or more of the following articles: Coping with Test Pain, Discomfort, and Anxiety, Tips on Blood Testing, Tips to Help Children through Their Medical Tests, and Tips to Help the Elderly through Their Medical Tests.
Another article, Follow That Sample, provides a glimpse at the collection and processing of a blood sample and throat culture.
Is any test preparation needed to ensure the quality of the sample?
No test preparation is needed; however, testing for diagnosis should not be performed after a recent blood transfusion.
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NOTE: This article is based on research that utilizes the sources cited here as well as the collective experience of the Lab Tests Online Editorial Review Board. This article is periodically reviewed by the Editorial Board and may be updated as a result of the review. Any new sources cited will be added to the list and distinguished from the original sources used.
Sources Used in Current Review
Raj, A. and Bertolone, S. (Updated 2009 July 9). Sickle Cell Anemia. eMedicine [On-line information]. Available online at http://emedicine.medscape.com/article/958614-overview through http://emedicine.medscape.com. Accessed September 2009.
Bender, M. A. and Hobbs, W. (Updated 2009 August 6). Sickle Cell Disease GeneReviews [On-line information]. Available online at http://www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=gene∂=sickle through http://www.ncbi.nlm.nih.gov. Accessed September 2009.
Hildreth, C. et. al. (2008 December 10). Sickle Cell Vasculopathy. JAMA. 2008;300(22):2690 [On-line information]. PDF available for download at http://jama.ama-assn.org/cgi/reprint/300/22/2690.pdf through http://jama.ama-assn.org. Accessed September 2009.
Mayo Clinic Staff (2009 April 1). Sickle cell anemia. MayoClinic.com [On-line information]. Available online at http://www.mayoclinic.com/health/sickle-cell-anemia/DS00324/METHOD=print through http://www.mayoclinic.com. Accessed September 2009.
(2008 February). Sickle Cell Disease. March of Dimes Fact Sheets [On-line information]. Available online at http://www.marchofdimes.com/professionals/14332_1221.asp through http://www.marchofdimes.com. Accessed September 2009.
(Reviewed 2009 June 9). Sickle Cell Disease: 10 Things You Need to Know. CDC [On-line information]. Available online at http://www.cdc.gov/Features/Sicklecell/ through http://www.cdc.gov. Accessed September 2009.
(2008 August). Sickle Cell Anemia. National Heart, Lung, and Blood Institute [On-line information]. Available online at http://www.nhlbi.nih.gov/health/dci/Diseases/Sca/SCA_WhatIs.html through http://www.nhlbi.nih.gov. Accessed September 2009.
Pagana, K. D. & Pagana, T. J. (© 2007). Mosby's Diagnostic and Laboratory Test Reference 8th Edition: Mosby, Inc., Saint Louis, MO. Pp 523-525.
Clarke, W. and Dufour, D. R., Editors (© 2006). Contemporary Practice in Clinical Chemistry: AACC Press, Washington, DC. Pp 219-223.
Wu, A. (© 2006). Tietz Clinical Guide to Laboratory Tests, 4th Edition: Saunders Elsevier, St. Louis, MO. Pp 518-521.
(October 5, 2009) National Guideline Clearinghouse. Screening for sickle cell disease in newborns: U.S. Preventive Services Task Force recommendation statement. Available online at http://www.guideline.gov/summary/summary.aspx?doc_id=11371&nbr=005908&string=sickle+AND+cell+AND+anemia through http://www.guideline.gov. Accessed October 2009.
Sources Used in Previous Reviews
(2006 May). What is Sickle Cell Anemia. National Heart, Lung and Blood Institute Information Center [On-line information]. Available online at http://www.nhlbi.nih.gov/health/dci/Diseases/Sca/SCA_WhatIs.html through http://www.nhlbi.nih.gov.
Bender, M.A. (2006 March 7, Updated). Sickle Cell Disease. GeneReviews [On-line information]. Available online through http://www.genetests.org.
(© 2006). Hemoglobin Evaluation. ARUP’s Guide to Clinical Laboratory Testing [On-line information]. Available online at http://www.aruplab.com/guides/clt/tests/clt_a307.jsp#1150784 through http://www.aruplab.com.
(2004). Interpretation of Newborn Hemoglobin Screening Results. Michigan Department of Community Health [On-line information]. Available online through http://www.michigan.gov.
(2004 August). Sickle Cell Disease. March of Dimes, Quick Reference Fact Sheets [On-line information]. Available online at http://www.marchofdimes.com/professionals/681_1221.asp through http://www.marchofdimes.com.