Hepatitis B Testing

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Also known as: HBV Tests; Hep B; anti-HBs; Hepatitis B Surface Antibody; HBsAg; Hepatitis B Surface Antigen; HBeAg; Hepatitis B e Antigen; anti-HBc; Hepatitis B Core Antibody; anti-HBc, IgM; anti-HBe; Hepatitis B e Antibody; HBV DNA
Formal name: Hepatitis B Virus Testing

At a Glance

Why Get Tested?

To screen for or diagnose a hepatitis B virus (HBV) infection or to determine if the vaccine against hepatitis B has produced the desired level of immunity; may also be used to guide treatment and assess its effectiveness

When to Get Tested?

When you have risk factors for HBV infection or when you have signs and symptoms of acute hepatitis; when you have a condition that requires chemotherapy or drugs that suppress your immune system and you might have been exposed to HBV in the past; when you are being treated for HBV

Sample Required?

A blood sample drawn from a vein in your arm

Test Preparation Needed?

None

The Test Sample

What is being tested?

Hepatitis B tests detect substances that reflect a current or previous infection with hepatitis B virus (HBV). Some tests detect viral proteins (antigens) or the antibodies that are produced in response to an infection, while other types of tests detect or evaluate the genetic material (DNA) of the virus. The pattern of test results can identify a person who has a current active infection or one who has immunity as a result of previous exposure.

For details on the various tests, see the table under "How is it used?"

Hepatitis is a condition characterized by inflammation and enlargement of the liver. It has several various causes, one of which is infection by a virus. HBV is one of five "hepatitis viruses" identified so far that are known to mainly infect the liver. The other four are hepatitis A, hepatitis C, hepatitis D, and hepatitis E.

HBV is spread through contact with blood or other body fluids from an infected person. Exposure can occur, for example, through sharing of needles for IV drug use or through unprotected sex. People who live in or travel to areas of the world where hepatitis B is prevalent are at a greater risk. Rarely, mothers can pass the infection to their babies, usually during or after birth. The virus is not spread through casual contact such as holding hands, coughing or sneezing. However, the virus can survive outside the body for up to seven days, including in dried blood, and can be passed by sharing items such as razors or toothbrushes with an infected person.

Effective hepatitis B vaccines have been available in the U.S. since 1981, and beginning in 1991, health care providers in the U.S. began vaccinating infants at birth. Still, the Centers for Disease Control and Prevention (CDC) estimates that between 804,000 and 1.4 million people in the U.S. are infected with the virus, most of whom are not aware that they are infected.

The course of HBV infections can vary from a mild form that lasts only a few weeks to a more serious chronic form lasting years. Sometimes chronic HBV leads to serious complications such as cirrhosis or liver cancer. Some of the various stages or forms of hepatitis B include:

  • Acute infection — presence of typical signs and symptoms with a positive screening test
  • Chronic infection — persistent infection with the virus detected by laboratory tests accompanied by inflammation of the liver
  • Carrier (inactive) state — persistent infection but no liver inflammation (a carrier is someone who may appear to be in good health but harbors the virus and can potentially infect others)
  • "Cleared" infection — no longer has any evidence of infection; viral antigen and DNA tests are negative and no signs or symptoms of liver inflammation (although, in many cases, the virus is present in an inactive state in the liver)
  • Reactivation — return of HBV infection with liver damage in a person who was a carrier or who had "cleared" infection; this most commonly occurs in persons treated with chemotherapy for cancer or with drugs that suppress the immune system used to treat autoimmune diseases or following an organ transplant.
  • Though a potentially serious infection, acute HBV infection usually resolves on its own in most adults. Infants and children tend to develop a chronic infection more often than adults. Approximately 90% of infants infected with HBV will develop a chronic condition. For children between the ages of one and five, the risk of developing chronic hepatitis drops to between 25% and 50%. Over the age of five, only 6% to 10% of HBV infections become chronic.

    The vast majority of those with chronic infections will have no symptoms. For acute infections, the symptoms are very similar to those of other types of acute hepatitis. Symptoms include fever, fatigue, nausea, vomiting, and jaundice. With acute hepatitis, the liver is damaged and is not able to function normally. It may not process toxins or waste products such as bilirubin for their removal from the body. During the course of disease, bilirubin and liver enzyme levels in the blood may increase. While tests such as bilirubin or a liver panel can tell a health practitioner that someone has hepatitis, they will not indicate what is causing it. Tests that detect infection with a hepatitis virus may help determine the cause.

    Hepatitis B testing can be used to screen for infection in the absence of symptoms, to determine whether infection is acute or chronic, or to monitor a chronic infection and the effectiveness of treatment. Initial testing may include the following, often performed together as a panel of tests:

    • Hepatitis B surface antigen
    • Hepatitis B surface antibody
    • Total hepatitis B core antibody (IgM and IgG)

    Additional or follow-up testing may include:

    • IgM antibody to hepatitis B core antigen
    • Hepatitis B e-antigen
    • Anti-hepatitis B e antibody
    • Hepatitis B viral DNA
    • Hepatitis B genotyping

    Two tests, hepatitis B surface Ag and hepatitis B core antibody, IgM, may be performed as part of an acute viral hepatitis panel.

    How is the sample collected for testing?

    A blood sample is drawn by needle from a vein in the arm.

    NOTE: If undergoing medical tests makes you or someone you care for anxious, embarrassed, or even difficult to manage, you might consider reading one or more of the following articles: Coping with Test Pain, Discomfort, and Anxiety, Tips on Blood Testing, Tips to Help Children through Their Medical Tests, and Tips to Help the Elderly through Their Medical Tests.

    Another article, Follow That Sample, provides a glimpse at the collection and processing of a blood sample and throat culture.

    Is any test preparation needed to ensure the quality of the sample?

    No test preparation is needed.

    The Test

    Common Questions

    Ask a Laboratory Scientist

    This form enables you to ask specific questions about your tests. Your questions will be answered by a laboratory scientist as part of a voluntary service provided by one of our partners, American Society for Clinical Laboratory Science. If your questions are not related to your lab tests, please submit them via our Contact Us form. Thank you.

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    Article Sources

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    NOTE: This article is based on research that utilizes the sources cited here as well as the collective experience of the Lab Tests Online Editorial Review Board. This article is periodically reviewed by the Editorial Board and may be updated as a result of the review. Any new sources cited will be added to the list and distinguished from the original sources used.

    Sources Used in Current Review

    Centers for Disease Control and Prevention. Hepatitis B Frequently Asked Questions. Available online at http://www.cdc.gov/hepatitis/b/bFAQ.htm#statistics through http://www.cdc.gov. Accessed August 18, 2013.

    Centers for Disease Control and Prevention. Viral Hepatitis Surveillance – U.S., 2010. Available online at http://www.cdc.gov/hepatitis/Statistics/2010Surveillance/Commentary.htm through http://www.cdc.gov. Accessed August 17, 2013.

    Centers for Disease Control and Prevention. 2012. The Pink Book: Course Textbook, 12th edition. Available online at http://www.cdc.gov/vaccines/pubs/pinkbook/hepb.html through http://www.cdc.gov. Accessed Aug 17, 2013.

    Hepatitis B Foundation. Hepatitis B Blood Test FAQ. Available online at http://www.hepb.org/patients/hepatitis_b_blood_tests.htm#ques1 through http://www.hepb.org. Accessed August 18, 2013.

    World Health Organization. Global alert and response. Hepatitis B. Available online at http://www.who.int/csr/disease/hepatitis/whocdscsrlyo20022/en/index3.html#serologicalmarkers through http://www.who.int. Accessed Aug 18, 2013.

    Fischbach, F.T., (2004) A Manual of Laboratory & Diagnostic Tests. 7th Edition., Lippincott Williams & Wilkins, Philadelphia.

    D. Robert Dufour. Viral Hepatitis: Overcoming Diagnostic Challenges. February 2014 Clinical Laboratory News: Volume 40, Number 2. Available online at http://www.aacc.org/publications/cln/2014/february/Pages/Viral-Hepatitis.aspx# through http://www.aacc.org. Accessed February 2014.

    Henry's Clinical Diagnosis and Management by Laboratory Methods. 22nd ed. McPherson R, Pincus M, eds. Philadelphia, PA: Saunders Elsevier: 2011, Pp 305-307.

    ©2014 American Red Cross. Learn About Blood, Blood Testing. Available online through http://www.redcrossblood.org. Accessed February 2014.

    Sources Used in Previous Reviews

    Clinical Chemistry: Principles, Procedures, Correlations. Michael L. Bishop, Janet L. Duben-Engelkirk, Edward P. Fody. Lipincott Williams & Wilkins, 4th Edition.

    Henry's Clinical Diagnosis and Management by Laboratory Methods. 21st ed. McPherson RA and Pincus MR, eds. Philadelphia: 2007, Pp 271-272.

    Clarke, W. and Dufour, D. R., Editors (2006). Contemporary Practice in Clinical Chemistry. AACC Press, Washington, DC, Pp 273-275, 491-492.

    American Association for the Study of Liver Diseases Practice Guidelines, Chronic Hepatitis B: Update 2009. PDF available for download at http://publish.aasld.org/practiceguidelines/Documents/Bookmarked%20Practice%20Guidelines/Chronic_Hep_B_Update_2009%208_24_2009.pdf through http://publish.aasld.org. Accessed January 2010.

    Centers for Disease Control and Prevention (Reviewed July 8, 2008). Hepatitis B information for Health Professionals. Available online at http://www.cdc.gov/hepatitis/HBV/index.htm through http://www.cdc.gov. Accessed January 2010.

    Centers for Disease Control and Prevention (July 19, 2008). Hepatitis B FAQs for the Public. Available online at http://www.cdc.gov/hepatitis/B/bFAQ.htm#overview through http://www.cdc.gov. Accessed January 2010.

    Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Burtis CA, Ashwood ER, Bruns DE, eds. St. Louis: Elsevier Saunders; 2006, Pp 1805-1806, 1809-1811.

    (Revised January 6, 2010) Hepatitis B Foundation, Diagnosis and Management. Available online at http://www.hepb.org/diagnosis/index.htm through http://www.hepb.org. Accessed January 2010.

    ArupConsult. Hepatitis B Testing and Testing Algorithm. Available online at http://www.arupconsult.com/Topics/InfectiousDz/Viruses/HBV.html# through http://www.arupconsult.com and PDF available for download at http://www.arupconsult.com/Algorithms/HBV.pdf. Accessed January 2010.

    MedlinePlus Medical Encyclopedia. Hepatitis B. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/000279.htm. Accessed January 2010.