Legionella Testing

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Also known as: Legionella pneumophila; Legionnaires Disease Testing
Formal name: Legionella Antigen, urine; Legionella Culture; Legionella by PCR

At a Glance

Why Get Tested?

To detect and diagnose Legionella bacteria as the cause of pneumonia or flu-like symptoms; to investigate an outbreak caused by Legionella

When to Get Tested?

When you have a cough, body aches, shortness of breath, headache, and fever with chills, and may have abdominal pain, diarrhea, nausea, and/or mental changes that a doctor suspects may be due to an infection caused by Legionella

Sample Required?

A urine sample, a sputum sample, and/or respiratory secretions or other body fluids collected by a doctor

Test Preparation Needed?


The Test Sample

What is being tested?

Legionella is a type of bacteria that can cause a serious form of pneumonia called Legionnaires disease. Legionella testing detects the presence of the bacteria to help diagnose the cause of a person's pneumonia.

A legionella infection affects the lower respiratory tract, often requires hospitalization, and has a significant mortality rate. In addition to pneumonia, Legionella bacteria can also cause Pontiac fever, a milder and self-limiting illness with flu-like symptoms. Together Legionnaires disease and Pontiac fever are referred to as legionellosis.

Legionella bacteria are found naturally throughout the environment. They prefer warm, stagnant water and can grow in the plumbing systems of large buildings such as hotels, hospitals, and cruise ships. The bacteria may contaminate whirlpool spas, drinking and bathing water, hot water tanks, air conditioning cooling towers, ice machines, humidifiers, and fountains.

Infections occur when an individual inhales steam, mist, or airborne droplets contaminated with Legionella bacteria. The infection is not spread from one person to another. Pontiac fever typically develops within 24-48 hours while Legionnaires disease develops within a few days to a couple of weeks. According to the Centers for Disease Control and Prevention (CDC), about 8,000 to 18,000 people are hospitalized with Legionnaires disease each year. The actual number of people affected by legionellosis is likely much higher as most cases of Pontiac fever and many cases of Legionnaires are thought to go undiagnosed.

Although anyone may be affected, adults over 50 are at the highest risk for Legionnaires disease, as are those who smoke, have lung diseases, or have compromised immune systems (such as those with HIV/AIDS, cancer, or who are taking immunosuppressant medications). People with chronic underlying diseases such as diabetes, kidney disease, or liver disease are also more vulnerable, and men are affected more often than women. Most cases of Legionnaires are sporadic, but they may also be seen in outbreaks, and Pontiac fever is frequently linked with outbreaks.

There are many different types of Legionella bacteria, but only a few are common. In the United States, as many as 80% of legionella infections in adults are caused by Legionella pneumophila, serogroup 1 (a subtype of the species). Other serogroups of L. pneumophila, including 4 and 6, cause a number of cases. Other species of Legionella, such as Legionella micdadei, Legionella bozemanii, Legionella dumoffii, and Legionella longbeachae, may cause infections in children and/or are more prevalent in other parts of the world.

A few different types of tests are available to help diagnose infections caused by Legionella bacteria:

  • An antigen test that detects a protein made by the bacteria in urine is one of the most common tests.
  • The bacteria may also be detected by culturing sputum, respiratory secretions or other body fluids.
  • A molecular test (polymerase chain reaction, PCR) may be used to identify Legionella in respiratory samples.

How is the sample collected for testing?

The sample collected depends on the test to be performed.

For culture or molecular testing, a sputum sample may be expectorated or induced. Expectorated samples are coughed up and expelled into a sterile cup provided by the laboratory or doctor's office. The person's mouth should be rinsed with water or saline prior to sample collection. Deep coughing is generally required, and the person should be informed that it is phlegm/mucus from the lungs that is necessary, not saliva. If someone cannot produce a sputum sample, then it can often be induced by inhaling a sterile saline or glycerin aerosol for several minutes to loosen phlegm in the lungs. Respiratory secretions, other body fluid samples, and any tissue biopsy samples are collected by a doctor.

For antigen testing, a random urine sample is collected in a sterile cup provided by the laboratory or doctor's office.

NOTE: If undergoing medical tests makes you or someone you care for anxious, embarrassed, or even difficult to manage, you might consider reading one or more of the following articles: Coping with Test Pain, Discomfort, and Anxiety, Tips on Blood Testing, Tips to Help Children through Their Medical Tests, and Tips to Help the Elderly through Their Medical Tests.

Another article, Follow That Sample, provides a glimpse at the collection and processing of a blood sample and throat culture.

Is any test preparation needed to ensure the quality of the sample?

No test preparation is needed.

The Test

Common Questions

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Article Sources

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NOTE: This article is based on research that utilizes the sources cited here as well as the collective experience of the Lab Tests Online Editorial Review Board. This article is periodically reviewed by the Editorial Board and may be updated as a result of the review. Any new sources cited will be added to the list and distinguished from the original sources used.

(Modified 2011 June 1). Patient Facts: Learn More about Legionnaires' disease. CDC Legionellosis Resource Site [On-line information]. Available online at http://www.cdc.gov/legionella/patient_facts.htm through http://www.cdc.gov. Accessed April 2012.

Mayo Clinic Staff (2010 December 10). Legionnaires' disease. MayoClinic.com [On-line information]. Available online at http://www.mayoclinic.com/print/legionnaires-disease/DS00853/METHOD=print&DSECTION=all through http://www.mayoclinic.com. Accessed April 2012.

Rathore, M. and Alvarez, A. (Updated 2011 October 25). Legionella Infection. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/965492-overview through http://emedicine.medscape.com. Accessed April 2012.

Cunha, B. and Sullivan, L. (Updated 2011 November 15). Legionnaires Disease. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/220163-overview through http://emedicine.medscape.com. Accessed April 2012.

Fisher, M. and Hillyard, D. (Updated 2011 November). Legionella pneumophila. ARUP Consult [On-line information]. Available online at http://www.arupconsult.com/Topics/Legionellapneumophila.html through http://www.arupconsult.com. Accessed April 2012.

Vorvick, L. (Updated 2011 February 19). Legionnaire's disease. MedlinePlus Medical Encyclopedia [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/000616.htm. Accessed April 2012.

Smeeks, F. (Updated 2011 March 31). Legionnaires Disease in Emergency Medicine. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/783656-overview through http://emedicine.medscape.com. Accessed April 2012.

Preidt, R. (2012 January 11). Outbreak of Legionnaires' Disease Traced to Hospital Fountain. MedlinePlus HealthDay [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/news/fullstory_120694.html through http://www.nlm.nih.gov. Accessed April 2012.

(Updated 2011 June 1). Top 10 Things Every Clinician Needs to Know About Legionellosis. CDC Legionellosis Resource Site [On-line information]. Available online at http://www.cdc.gov/legionella/top10.htm through http://www.cdc.gov. Accessed April 2012.

Hadjiliadis, D. (Updated 2010 September 15). Atypical pneumonia. MedlinePlus Medical Encyclopedia [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/000079.htm through http://www.nlm.nih.gov. Accessed April 2012.

Dugdale, D. (Updated 2010 May 23). Sputum direct fluorescent antibody (DFA). MedlinePlus Medical Encyclopedia [On-line information]. Available online at http://www.nlm.nih.gov/medlineplus/ency/article/003553.htm. Accessed April 2012.

Hicks, L. and Garrison, L. (Updated 2011 July 1). Legionellosis (Legionnaires' Disease & Pontiac Fever). CDC Travelers' Health, Chapter 3 Infectious Diseases Related To Travel [On-line information]. Available online at http://wwwnc.cdc.gov/travel/yellowbook/2012/chapter-3-infectious-diseases-related-to-travel/legionellosis-legionnaires-disease-and-pontiac-fever.htm through http://wwwnc.cdc.gov. Accessed April 2012.

(Modified 2011 June 6). Travel-Associated Legionnaires' Disease For Health Departments: Common Q&As CDC Legionellosis Resource Site [On-line information]. Available online at http://www.cdc.gov/legionella/faq.htm through http://www.cdc.gov. Accessed April 2012.

Bartram, J. et. al. Editors (© 2007). Legionella and the Prevention of Legionellosis World Health Organization [On-line information]. PDF available for download at http://www.who.int/water_sanitation_health/emerging/legionella.pdf through http://www.who.int. Accessed April 2012.

Pagana, K. D. & Pagana, T. J. (© 2011). Mosby's Diagnostic and Laboratory Test Reference 10th Edition: Mosby, Inc., Saint Louis, MO. Pp 616.

Forbes, B. et. al. (© 2007). Bailey & Scott's Diagnostic Microbiology, 12th Edition: Mosby Elsevier Press, St. Louis, MO. Pp 424-429.