ANCA/MPO/PR3 Antibodies

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Also known as: ANCA Antibodies; c-ANCA; p-ANCA; Serine Protease 3; MPO; PR3; Anticytoplasmic Autoantibodies; 3-ANCA; PR3-ANCA; MPO-ANCA
Formal name: Antineutrophil Cytoplasmic Antibodies; Myeloperoxidase Antibodies; Proteinase 3 Antibodies
Related tests: Autoantibodies; CBC; ESR; Urinalysis

At a Glance

Why Get Tested?

To test for certain autoimmune disorders, such as Wegener's granulomatosis (WG), microscopic polyangitis (MPA), and a number of others

When to Get Tested?

When your doctor thinks that you have symptoms that may be due to a vascular autoimmune disorder; sometimes to monitor response to therapy

Sample Required?

A blood sample drawn from a vein in your arm

Test Preparation Needed?


The Test Sample

What is being tested?

Antineutrophil cytoplasmic antibodies (ANCA) are a group of autoantibodies produced when a person's immune system mistakenly targets and attacks its own neutrophil proteins. Two of the most commonly targeted proteins are myeloperoxidase (MPO) and proteinase 3 (PR3). This results in the production of antibodies to MPO and/or PR3. The ANCA blood test detects the presence or absence of these autoantibodies by looking at the pattern of fluorescence on a slide under the microscope.

Antineutrophil cytoplasmic antibodies may be present in a variety of autoimmune disorders that cause inflammation and damage to blood vessels throughout the body (systemic vasculitis). Vasculitis can cause tissue and organ damage due to the narrowing and obstruction of blood vessels and the subsequent loss of blood supply. It can also produce areas of weakness in blood vessel walls, known as aneurysms, which have the potential to rupture.

The symptoms experienced by a person with systemic vasculitis depend upon the degree of autoimmune activity and the parts of the body involved. Early in the disease process, symptoms are often nonspecific – they include fatigue, fever, weight loss, muscle aches, and night sweats. As the disorder progresses, vascular damage may affect the functioning of the kidneys, eyes, skin, lungs, and liver. This can cause a wide range of symptoms related to these organ systems.

PR3 antibodies are most frequently seen in Wegener's granulomatosis (WG). MPO antibodies are most often associated with microscopic polyangiitis but may also be seen in people with pauci-immune necrotizing glomerulonephritis, Churg-Strauss syndrome, and WG. For more about these conditions, visit the Vasculitis Foundation.

How is the sample collected for testing?

A blood sample is obtained by inserting a needle into a vein in the arm.

NOTE: If undergoing medical tests makes you or someone you care for anxious, embarrassed, or even difficult to manage, you might consider reading one or more of the following articles: Coping with Test Pain, Discomfort, and Anxiety, Tips on Blood Testing, Tips to Help Children through Their Medical Tests, and Tips to Help the Elderly through Their Medical Tests.

Another article, Follow That Sample, provides a glimpse at the collection and processing of a blood sample and throat culture.

Is any test preparation needed to ensure the quality of the sample?

No test preparation is needed.

The Test

Common Questions

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Article Sources

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NOTE: This article is based on research that utilizes the sources cited here as well as the collective experience of the Lab Tests Online Editorial Review Board. This article is periodically reviewed by the Editorial Board and may be updated as a result of the review. Any new sources cited will be added to the list and distinguished from the original sources used.

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