Most often, this test is done to measure the strength of your immune system if you have been diagnosed with human immunodeficiency virus (HIV) infection and to monitor the effectiveness of treatment; occasionally, it may be used with other conditions (see Common Questions below).
CD4 Count
CD4 Count
Also Known As
T4 Count
T-helper Cells
CD4 Percent
Formal Name
CD4 Lymphocyte Count
This article was last reviewed
on
This article waslast modified
on July 15, 2019.
At a Glance
Why Get Tested?
When To Get Tested?
When you are first diagnosed with HIV infection to get a baseline assessment of your immune system; about every 3 to 6 months after starting antiretroviral therapy (ART) to check whether you are responding to treatment; if you have responded well to treatment, you may then be tested every 6 to 12 months.
Sample Required?
A blood sample drawn from a vein in your arm
Test Preparation Needed?
None
Accordion Title
Common Questions
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How is the test used?
CD4 counts, along with an HIV viral load, are most often used if you are diagnosed with HIV infection to:
- Predict whether the disease will worsen over time (progress)
- Determine how quickly to start treatment or the need for treatment to prevent opportunistic infections (prophylaxis)
- Evaluate the immune system
- Monitor effectiveness of antiretroviral treatment (ART or ARV), also called highly active antiretroviral therapy (HAART)
It is recommended that all individuals diagnosed with HIV infection receive antiretroviral treatment as soon as possible, including pregnant women, to reduce the risk of disease progression. People typically take at least three drugs from two different classes in order to prevent or minimize virus replication and the emergence of drug-resistant strains. Combinations of three or more antiretroviral drugs are referred to as highly active antiretroviral therapy or HAART.CD4 counts are most useful when they are compared with results obtained from earlier tests. Since CD4 cells are usually destroyed more rapidly than other types of lymphocytes and because absolute counts can vary from day to day, it is sometimes useful to look at the number of CD4 cells compared to the total lymphocyte count. The result is expressed as a percentage, i.e., CD4 percent.
Sometimes, CD4 tests may be used to help diagnose or monitor other conditions such as lymphoma, organ transplantation, and DiGeorge syndrome (see below).
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When is it ordered?
A CD4 count is usually ordered along with an HIV viral load when you are first diagnosed with HIV infection as part of a baseline measurement. After the baseline, a CD4 count will usually be ordered at intervals over time, depending on a few different factors.
The following table summarizes recommendations* for the timing of CD4 counts and viral load testing:
Clinical Status of Patient Viral Load CD4 Count When first diagnosed Test performed Test performed If ART is delayed Optional Every 3-6 months After initiating ART Within 2-4 weeks and then every 4-8 weeks until virus is suppressed (undetectable) 3 months later During the first 2 years of stable ART Every 3-4 months Every 3-6 months Virally suppressed on ART for greater than 2 years Can extend to every 6 months Annually; if CD4 consistently greater than 500 cells/mm3, monitoring is optional While on ART and viral load is consistently greater than 200 copies/mL Every 3 months Every 3-6 months With new HIV symptoms or start of new treatment with interferon, corticosteroids or cancer drugs Every 3 months Perform test and monitor according to health status (e.g., new HIV symptoms, opportunistic infections) *Adapted from Guidelines for the Use of Antiretroviral Agents in HIV-1–Infected Adults and Adolescents, Table 4. Recommendations on the Indications and Frequency of Viral Load and CD4 Count Monitoring.
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What does the test result mean?
A CD4 count is typically reported as a count of cells (expressed as cells per cubic millimeter of blood). Sometimes results are expressed as a percent of total lymphocytes (CD4 percent).
- A normal CD4 count ranges from 500–1,200 cells/mm3 in adults and teens. In general, a normal CD4 count means that your immune system is not yet significantly affected by HIV infection.
- A low CD4 count indicates that your immune system has been affected by HIV and/or the disease is progressing. At CD4 counts less than 200 cells/mm3, the immune system can no longer keep opportunistic infections in check. At low CD4 counts, a healthcare practitioner may recommend starting prophylactic treatment for opportunistic infections such as Pneumocystis carinii (jiroveci) pneumonia (PCP) or candidiasis (thrush). The Centers for Disease Control and Prevention (CDC) considers people who have an HIV infection and CD4 counts below 200 cells/mm3 to have AIDS (stage III HIV infection), regardless of whether they have any signs or symptoms.
Once the virus has been suppressed due to ART, CD4 counts typically rise and/or stabilize over time, indicating that you are responding to treatment. Within the first year of treatment it is normal for the CD4 count to increase by 50-150 cells/mm3. However, a slower change in the CD4 count is typically not sufficient to change treatment. It is rare for the CD4 count to decline when the virus is suppressed due to ART.Importantly, any single CD4 test result may differ from the last one even though your health status has not changed. Usually, a healthcare practitioner will take several CD4 test results into account rather than a single value and will evaluate the pattern of CD4 results over time.
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Is there anything else I should know?
The CD4 count tends to be lower in the morning and higher in the evening. Acute illnesses, such as pneumonia, influenza, or herpes simplex virus infection, can cause the CD4 count to decline temporarily. Cancer chemotherapy can dramatically lower the CD4 count.
The CD4 count does not always reflect how someone with HIV disease feels and functions. For example, some people with higher counts are ill and have frequent complications, and some people with lower CD4 counts have few medical complications and function well.
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How is HIV infection diagnosed?
HIV infection is usually screened for with a test for HIV antibody and antigen (p24). If the screening test is positive, it must be followed with another test, such as a second antibody test that can differentiate HIV-1 and HIV-2. If results of the first and second test do not agree, then an HIV-1 RNA test (nucleic acid amplification test, NAAT) is performed. If either the second antibody test or the HIV-1 RNA is positive, then you are diagnosed with HIV infection. Read the article on HIV Antibody and HIV Antigen (p24) for more details.
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What are some common opportunistic infections I might get if I have an HIV infection?
Opportunistic infections are those that occur more frequently in people who have a weakened immune system. For people infected with HIV, a CD4 count less than 200 cells/mm3 puts you at risk for opportunistic infections, including pneumocystis pneumonia, toxoplasma encephalitis, cytomegalovirus (CMV) retinitis, tuberculosis and disseminated infections caused by nontuberculosis mycobacteria, cryptococcal meningitis, as well as candidiasis and other fungal infections. The Centers for Disease Control and Prevention (CDC) provides a table with examples of common opportunistic infections. Viral suppression virtually eliminates this risk.
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What are my treatment options?
In general, treatment is recommended regardless of whether the CD4 count is low or within the normal range. You and your healthcare provider should discuss your treatment options to determine what will work best for you. The Mayo Clinic web page HIV/AIDS: Treatments and drugs has detailed information on various therapies.
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Is a CD4 cell count used in conditions other than HIV infection?
Yes. It may be ordered when a person has had an organ transplant to help evaluate the effect of immunosuppressive medications. In transplantation, the immune system must be suppressed so that it does not attack the transplanted organ and cause rejection. In this case, it is desirable to have low levels of CD4 cells, and a decreased count shows that the drug is working. A CD4 count may be repeated periodically to monitor the effectiveness of therapy.
CD4 counts are sometimes done in conjunction with CD8 counts. CD8 cells are another type of lymphocyte called T-suppressor cell or cytotoxic T cell. CD8 cells identify and kill cells that have been infected with viruses or that have been affected by cancer.
Evaluation of CD4 and CD8 cells may be used to help classify lymphomas. Typically, several markers on the surface of lymphocytes in addition to CD4 and CD8 are evaluated. The tests help determine whether the lymphoma is due to the proliferation of B lymphocytes or T lymphocytes and which specific type. This information is useful in determining appropriate therapy.
These tests may also help diagnose DiGeorge syndrome, a rare congenital disorder characterized by, among other things, low levels of T cells in the blood. For more information on DiGeorge syndrome, visit the Mayo Clinic web site.
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