Also Known As
Epidermal Growth Factor Receptor Mutation Analysis
HER-1
erbB-1
Formal Name
Epidermal Growth Factor Receptor
This article was last reviewed on
This article waslast modified on September 21, 2018.

Were you looking instead for Estimated Glomerular Filtration Rate, also known as eGFR? If so, see the article on eGFR.

At a Glance
Why Get Tested?

To detect EGFR gene mutations in non-small cell lung cancer tumor cells; knowing whether a mutation is present in the cells can help determine if EGFR-targeted tyrosine kinase inhibitors such as gefitinib and erlotinib may be beneficial for treating the tumor.

When To Get Tested?

When you have been diagnosed with non-small cell lung cancer; when you are being treated with an EGFR-targeted tyrosine kinase inhibitor, but the cancer progresses

Sample Required?

A sample of cancer cells from a biopsy or surgical removal of cancerous tissue; if insufficient cells are obtained for testing by one of these methods, then a blood sample may be used as an alternative in some situations.

Test Preparation Needed?

In many cases preparation is minimal, but it will depend upon how the cancer cells are being obtained. You will receive instructions from your healthcare practitioner based on the procedure being used to obtain the sample.

You may be able to find your test results on your laboratory's website or patient portal. However, you are currently at Lab Tests Online. You may have been directed here by your lab's website in order to provide you with background information about the test(s) you had performed. You will need to return to your lab's website or portal, or contact your healthcare practitioner in order to obtain your test results.

Lab Tests Online is an award-winning patient education website offering information on laboratory tests. The content on the site, which has been reviewed by laboratory scientists and other medical professionals, provides general explanations of what results might mean for each test listed on the site, such as what a high or low value might suggest to your healthcare practitioner about your health or medical condition.

The reference ranges for your tests can be found on your laboratory report. They are typically found to the right of your results.

If you do not have your lab report, consult your healthcare provider or the laboratory that performed the test(s) to obtain the reference range.

Laboratory test results are not meaningful by themselves. Their meaning comes from comparison to reference ranges. Reference ranges are the values expected for a healthy person. They are sometimes called "normal" values. By comparing your test results with reference values, you and your healthcare provider can see if any of your test results fall outside the range of expected values. Values that are outside expected ranges can provide clues to help identify possible conditions or diseases.

While accuracy of laboratory testing has significantly evolved over the past few decades, some lab-to-lab variability can occur due to differences in testing equipment, chemical reagents, and techniques. This is a reason why so few reference ranges are provided on this site. It is important to know that you must use the range supplied by the laboratory that performed your test to evaluate whether your results are "within normal limits."

For more information, please read the article Reference Ranges and What They Mean.

What is being tested?

EGFR is a short name for the Epidermal Growth Factor Receptor gene. DNA from tumor cells is tested for mutations in this gene. In certain situations, DNA that has been shed from tumor cells in one's blood can also be tested and may be informative.

The EGFR gene provides instructions for a cell to make a specialized type of protein called a receptor, which is located on the surface of the cell. A receptor recognizes and binds to other substances, and the binding typically has a specific effect on the cells. EGFR is one of a group of receptors called receptor tyrosine kinases that help regulate cell growth, division, survival, and death. This regulation is a very complex process. Receptor tyrosine kinases like EGFR transmit signals from the surface of the cell to pathways within the cell.

In normal cells, binding of epidermal growth factor to its receptor on the cell surface is an important signal that can promote cell growth and proliferation. Certain mutations called "activating mutations" in the EGFR gene can result in excessive signaling for growth and uncontrolled proliferation of cancer cells.

Treatments that target EGFR are an effective therapy for certain cancers. Examples of EGFR-targeted therapy include drugs such as gefitonib and erlotinib, which are small molecules called tyrosine kinase inhibitors (TKIs) that enter cancer cells and impede signaling through the EGFR pathway. These drugs have been shown to be useful in treating non-small cell lung cancer (NSCLC), if the tumor has a sensitive EGFR mutation. If a sensitive mutation is detected with an EGFR mutation test, then EGFR-targeted tyrosine kinase inhibitors are much more likely to be of benefit for treating the tumor.

Accordion Title
Common Questions
  • How is it used?

    This test is helpful to direct therapy. It detects the presence of mutations in the EGFR gene in the DNA of the tumor cells to help determine whether someone with non-small cell lung cancer (NSCLC) may benefit from targeted therapy with tyrosine kinase inhibitors (TKIs) such as gefitinib or erlotinib. Activating EGFR mutations can lead to uncontrolled growth of cancer cells. TKIs can block the action of EGFR to inhibit cancer cell growth.

    An EGFR mutation test may be ordered by itself or as part of a panel (a series of tests to detect mutations in other genes such as KRAS, ALK and ROS1). Each of these tests may be used to help determine whether a person's lung cancer will respond to targeted therapy and which type will be of more benefit.

  • When is it ordered?

    EGFR mutation testing is ordered after an individual has been diagnosed with non-small cell lung cancer, especially adenocarcinoma.

    If a patient has already been treated with an EGFR-targeted tyrosine kinase inhibitor but does not appear to be responding favorably to the treatment, the healthcare practitioner may order additional EGFR mutation testing on a new sample, to check for specific EGFR mutations that may account for lack of a favorable response. A new sample of tumor tissue is required to determine whether the cancer has acquired new mutations that make it resistant to the current treatment. For example, one of the known resistance mutations, specifically T790M, may be detected and can then be targeted with a different drug that has been designed especially for this particular mutation.

  • What does the test result mean?

    If an individual's lung cancer has an activating EGFR mutation, then an EGFR-targeted tyrosine kinase inhibitor (TKI) may be effective in treating the tumor. If no mutation is detected by the EGFR test, then the tumor is less likely to respond to TKI therapy. Sometimes an EGFR mutation is detected, but it indicates that the cancer will not respond to a TKI. Pathologists and cancer specialists (oncologists) help interpret the precise significance of the exact EGFR mutation identified for each patient.

  • Is there anything else I should know?

    EGFR mutation testing is usually optimized to detect EGFR mutations that have demonstrated significance, but there may be uncommon or unique EGFR mutations present in a tumor that are not detected.

    Although a mutation is present in the tumor cells, if there is very little tumor tissue and/or when there are insufficient tumor cells present that contain the mutation, the test may result in "no mutation detected" despite the mutation being present in a minority of tumor cells. Because the amount of tumor cells or tumor DNA in a blood sample is uncertain, testing a blood sample (also called a liquid biopsy) is generally not preferred.

    The well-known activating EGFR mutations typically result in changes to small areas of EGFR protein. Testing for these types of mutations is commonly done by techniques such as real-time PCR or next-generation sequencing.

  • What other testing might be done?

    ALK and/or ROS1 mutation testing are recommended to determine whether a person's tumor would be likely to respond to specific targeted therapies that are effective when an ALK or ROS1 mutation is detected. Testing for mutations in other genes (e.g., KRAS) may be offered for lung cancer patients if there is enough tissue sample available.

  • How long does it take for EGFR testing results?

    EGFR mutation testing is not available in every laboratory. If a healthcare practitioner sends a sample to a reference laboratory and depending on the test method used, the results may take a few days to several days before they are available.

  • Can I have my blood tested for EGFR mutations?

    Yes, but testing blood may give false-negative results because the amount of tumor cell DNA in the blood is uncertain. Therefore, a biopsy or surgical sample of the tumor is generally preferred. When tissue from a biopsy or surgical sample is limited, unavailable, and/or insufficient for molecular testing, physicians may try a blood sample instead. If the blood result isn't informative, then testing a sample from the tumor is necessary. Also, patients may wish to consult their health insurance company as to whether this blood testing would be covered.

  • What is the difference between EGFR (also known as HER-1) and HER-2?

    EGFR and HER-2 are both members of the same family of cell membrane receptors. When either EGFR or HER-2 is significantly increased in a cancer, it often indicates a more aggressive tumor and a poorer patient prognosis. The primary differences between EGFR and HER-2 testing are that they are ordered to help evaluate different types of cancer and that the drugs that have been developed to target them are specific for that particular receptor, EGFR or HER-2. HER-2 is evaluated in breast cancer and cancer of the stomach and esophagus (gastroesophageal adenocarcinoma).

  • Are there other types of testing that evaluate EGFR in tumors?

    There are tests available that identify other changes in EGFR known as gene amplification, which means an abnormally increased number of copies of the EGFR gene are present in the tumor cells. Another type of test looks at the amount of EGFR protein present on the tumor cells. These tests are not helpful for identifying patients with lung cancer who may benefit from EGFR-targeted tyrosine kinase inhibitor therapy. However, they may have a role in other types of cancer. For example, sometimes tests for EGFR gene amplification are used to determine the likely course (prognosis) of cancer of the head and neck.

  • Besides lung cancer, are there other types of cancers that have increased EGFR protein?

    Other types of cancer that may have increased EGFR protein present include colon cancer, glioblastoma (a type of brain cancer), and cancers of the head and neck, breast, and pancreas. EGFR blocking agents are routinely used for treatment of metastatic colon cancer and are used with some head and neck cancers. The utility of EGFR inhibitors and their correlation with EGFR mutations in different types of cancers hasn't been fully established but continues to be investigated.

View Sources

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