Also Known As
Qualitative or Quantitative Stool Fat
Stool Lipids
72 Hour Fecal Fat
Fat Stain Oil Red O
Fat, Feces
Formal Name
Fat, Fecal Qualitative or Quantitative
This article was last reviewed on
This article waslast modified on July 2, 2020.
At a Glance
Why Get Tested?

To detect and measure excess fat in the stool; to help diagnose conditions causing malabsorption

When To Get Tested?

When you have symptoms of malabsorption, such as persistent diarrhea and fatty stools

Sample Required?

A random stool collection; sometimes a 72-hour stool collection

Test Preparation Needed?

For a 72-hour stool collection, follow your healthcare practitioner's instructions; this may include consuming 50-150 grams of fat a day in your diet for 2-3 days prior to and during the stool collection period.

You may be able to find your test results on your laboratory's website or patient portal. However, you are currently at Lab Tests Online. You may have been directed here by your lab's website in order to provide you with background information about the test(s) you had performed. You will need to return to your lab's website or portal, or contact your healthcare practitioner in order to obtain your test results.

Lab Tests Online is an award-winning patient education website offering information on laboratory tests. The content on the site, which has been reviewed by laboratory scientists and other medical professionals, provides general explanations of what results might mean for each test listed on the site, such as what a high or low value might suggest to your healthcare practitioner about your health or medical condition.

The reference ranges for your tests can be found on your laboratory report. They are typically found to the right of your results.

If you do not have your lab report, consult your healthcare provider or the laboratory that performed the test(s) to obtain the reference range.

Laboratory test results are not meaningful by themselves. Their meaning comes from comparison to reference ranges. Reference ranges are the values expected for a healthy person. They are sometimes called "normal" values. By comparing your test results with reference values, you and your healthcare provider can see if any of your test results fall outside the range of expected values. Values that are outside expected ranges can provide clues to help identify possible conditions or diseases.

While accuracy of laboratory testing has significantly evolved over the past few decades, some lab-to-lab variability can occur due to differences in testing equipment, chemical reagents, and techniques. This is a reason why so few reference ranges are provided on this site. It is important to know that you must use the range supplied by the laboratory that performed your test to evaluate whether your results are "within normal limits."

For more information, please read the article Reference Ranges and What They Mean.

What is being tested?

This test measures the amount of fat in a stool sample. Excess fecal fat (termed steatorrhea) may be an indication that a person's digestive system is not working properly and/or that the individual has a condition affecting the digestion of food and absorption of nutrients (malabsorption).

The body digests foods in three stages: first proteins, fats, and carbohydrates are broken down, in the stomach by acid and enzymes and in the small intestines by enzymes produced by the pancreas and bile from the liver, into their component parts. They are then absorbed, primarily in the small intestines. Finally, the nutrients are transported throughout the body and used or stored.

If there are not enough pancreatic enzymes or bile available, then fat and other foods cannot be properly digested. If a condition prevents the intestines from absorbing nutrients, then they are "lost" by excretion in the stool. In both cases – improper digestion or absorption – the affected person can experience symptoms associated with malabsorption and, in severe cases, symptoms of malnutrition and vitamin deficiency. If the condition prevents the digestion and/or absorption of fats from the diet, then excess fat is present in the stool and the person may experience prolonged diarrhea with stomach pain, cramps, bloating, gas, and weight loss.

Fat in stool may be detected with the qualitative fecal fat test, which generally determines the presence or absence of excess fat. This is the simplest test for fecal fat and is performed by placing a suspension of treated or untreated stool onto a glass slide, adding a fat stain, and observing the number and size of fat globules that are present.

Quantitative measurements of fecal fat, though somewhat more precise, are less commonly performed. They require a timed stool collection and a dietary sheet to help calculate the total fat intake during the collection period, usually a 72-hour stool collection. Results are reported as the amount of fat excreted per 24 hours. A variation of the test is called the acid steatocrit, which provides a rapid but less exact measure of the amount of fat in the stool.

How is the sample collected for testing?

For a 72-hour stool collection, save all stool during the collection time period in the container(s) provided. It may be collected in a variety of ways, such as on plastic wrap, but should not be contaminated by urine, toilet water, or toilet paper. For a random sample, collect a single specimen in the same fashion.

Is any test preparation needed to ensure the quality of the sample?

For a 72-hour stool collection, follow your healthcare provider's instructions and dietary recommendations. This may include consuming a fat-controlled diet containing 50-150 grams of fat a day for 2-3 days prior to and during the stool collection period. You will also be asked to avoid certain oils and fat substitutes during the collection as these can invalidate the test result.

Accordion Title
Common Questions
  • How is it used?

    Fecal fat testing is typically ordered along with or as a follow up to other stool tests and blood tests to investigate the cause of chronic diarrhea and loose, fatty, foul-smelling stools (steatorrhea).

    Stool tests may include:

    • Fecal occult blood test (FOBT) – to detect blood in the stool
    • O&P (Ova and Parasite) – to detect parasites
    • Fecal white blood cells – to detect white blood cells in the stool
    • Fecal elastase – a protein-cleaving enzyme produced and secreted by the pancreas; it is resistant to degradation by other enzymes and so is excreted and can be measured in the stool. The amount of this enzyme is reduced in pancreatic insufficiency.
    • Fecal fat, qualitative – usually a suspension of stool placed on a glass slide that is treated with a special stain and examined under the microscope to detect the presence of fat droplets

    Blood tests may be ordered to further investigate malabsorption symptoms and may include:

    Tests for cystic fibrosis may be performed as part of the investigation:

    If the qualitative fecal fat is negative, then a 72-hour quantitative fecal fat test may be ordered. This is a better evaluation of fat digestion and absorption. There are two reasons for this:

    • For the quantitative test, the person being tested is required to ingest a moderately high amount of fat per day prior to and during sample collection so their absorption ability is being "challenged."
    • Fat is not released into the stool at a constant rate so the combination and mixing of the stool from a 72-hour collection gives a more accurate picture of average absorption and elimination than a single sample.
  • When is it ordered?

    A fecal fat test is primarily ordered when a person has signs and symptoms of malabsorption such as:

    • Fatty stools that are loose and foul-smelling (steatorrhea)
    • Persistent diarrhea
    • Abdominal pain, cramps, bloating, and gas
    • Weight loss
    • Failure to thrive (in children)

    These symptoms may or may not be accompanied by other indicators such as fatigue, anemia and/or specific nutritional deficiencies in, for example, iron or vitamin B12.

    A qualitative fecal fat may be ordered as a screening test. If it is positive for excess fecal fat, then a quantitative test is generally not necessary.

    If the qualitative test is negative and the healthcare provider still suspects excess fecal fat, then a quantitative 72-hour fecal fat may be ordered.

  • What does the test result mean?

    A positive qualitative fecal fat test or an increased amount of fat in a 72-hour quantitative fecal fat test indicates that fat is likely not being absorbed normally and that the person may have impaired digestion or malabsorption.

    Malabsorption is seen with a wide variety of diseases and conditions. Some causes of malabsorption include:

    Other laboratory tests used in conjunction with the fecal fat test are usually required to determine the underlying cause of fat malabsorption.

    A negative qualitative fecal fat test does not necessarily rule out malabsorption so it may be followed up with a quantitative test.

    In a 72-hour fecal fat test, a low level of fecal fat generally indicates that the person tested is digesting and absorbing fats normally and suggests that the symptoms being experienced are likely due to another cause.

  • Is there anything else I should know?

    Laxatives, enemas, barium, mineral oil, fat-blocking supplements, psyllium fiber, and fat substitutes may affect test results.

    Children cannot ingest as much daily fat as adults. Their test preparation will be adjusted and their 72-hour fecal fat test results will typically be reported as a percentage. This result is a "coefficient" that compares the amount of fat eaten to the amount excreted in order to evaluate the quantity of fat absorbed.

    Although 72 hours is the typical sample collected for a quantitative fecal fat, a healthcare provider may sometimes ask for a 24- or 48-hour stool sample instead. 

  • If my doctor tells me to eat 100 grams of fat a day, can I vary it between 50 and 150 grams?

    You should try to follow your healthcare provider's recommendations as closely as possible. Eating a similar amount of fat each day will help ensure that the interpretation of your results is accurate. For more information on the fat content of various foods, visit the page on Food Exchange Lists by the National Heart, Lung and Blood Institute.

  • Can I just do the screening fecal fat test and not the 72-hour test?

    If the screening test is positive, then the 72-hour test is not generally necessary. However, if it is negative, then your healthcare provider may want the additional information that the 72-hour sample provides.

  • Can the fecal fat test be done in my doctor's office?

    The qualitative fecal fat test may be done in a healthcare provider's office if it has the proper stain and equipment. The quantitative test requires specialized equipment; it needs to be performed by a laboratory and may need to be sent to a reference laboratory.

Health Professionals - Optimal Testing Recommendations

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**This content is intended for healthcare professionals.**

Visit the For Health Professionals: 72 Hour Quantitative Fecal Fat article for the full recommendations.

  • A 72 hour timed quantitative fecal fat measurement is not indicated in the differentiation of pancreatic diseases and should be used with caution in the evaluation of malabsorption.
  • Although referenced as a gold standard for the diagnosis of malabsorption, often, results from 72 hour quantitative fecal fat testing do not provide significant clinical value, and test results do not provide additional information on the causation of malabsorption.
  • 72 hour quantitative fecal fat test results are highly sensitive to pre-analytical considerations (i.e., dietary requirements), which may significantly impact the test interpretation.

The content for Optimal Testing: AACC's Guide to Lab Test Utilization has been developed and approved by the AACC Academy and AACC's Science and Practice Core Committee.

View Sources

Sources Used in Current Review

Lehrer, J. (2014 August 19 Updated). Fecal fat. MedlinePlus Medical Encyclopedia [On-line information]. Available online at Accessed on 10/08/15.

(2015 July 14 Updated). Short Bowel Syndrome. National Institute of Diabetes and Digestive and Kidney Diseases [On-line information]. Available online at Accessed on 10/08/15.

Goebel, S. (2014 December 16 Updated). Malabsorption. Medscape Drugs & Diseases [On-line information]. Available online at Accessed on 10/08/15.

Stefano Guandalini, S. et. al. (2015 July 17 Updated). Pediatric Malabsorption. Medscape Drugs & Diseases [On-line information]. Available online at Accessed on 10/08/15.

Delgado, J. and Grenache, D. (2015 September Updated). Malabsorption. ARUP Consult [On-line information]. Available online at Accessed on 10/08/15.

Ruiz, A. (2014 May Revised). Overview of Malabsorption. Merck Manual Professional Version [On-line information]. Available online at Accessed on 10/08/15.

Sources Used in Previous Reviews

Dugdale, D. (Updated 2010 August 14) Fecal Fat. MedlinePlus Medical Encyclopedia [On-line information]. Available online at Accessed June 2011.

(© 1995-2011). Unit Code 8310: Fat, Feces. Mayo Clinic Mayo Medical Laboratories [On-line information]. Available online at Accessed June 2011.

Dugdale, D. (Updated 2010 July 7). Malabsorption. MedlinePlus Medical Encyclopedia [On-line information]. Available online at Accessed June 2011.

Delgado, J. and Grenache, D. (Updated 2010 November). Malabsorption. ARUP Consult [On-line information]. Available online at Accessed June 2011.

Ruiz, A. (Revised 2008 January). Malabsorption. Merck Manual for Healthcare Professionals [On-line information]. Available online at Accessed June 2011.

Pagana, K. D. & Pagana, T. J. (© 2011). Mosby's Diagnostic and Laboratory Test Reference 10th Edition: Mosby, Inc., Saint Louis, MO. Pp 441-442.

Clarke, W. and Dufour, D. R., Editors (© 2006). Contemporary Practice in Clinical Chemistry: AACC Press, Washington, DC. Pp 304-305.

Wu, A. (© 2006). Tietz Clinical Guide to Laboratory Tests, 4th Edition: Saunders Elsevier, St. Louis, MO. Pp 384-387.

Henry's Clinical Diagnosis and Management by Laboratory Methods. 21st ed. McPherson R, Pincus M, eds. Philadelphia, PA: Saunders Elsevier: 2007, Pp 291-292.

Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. Burtis CA, Ashwood ER, Bruns DE, eds. St. Louis: Elsevier Saunders; 2006, Pp 1878-1881.

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