To diagnose an infection with Helicobacter pylori (H. pylori), the bacteria that can cause peptic ulcers; to determine whether treatment has cured the infection
Helicobacter pylori (H. pylori) Testing
When you have symptoms of an ulcer, such as ongoing indigestion, gastrointestinal pain that comes and goes, unexplained weight loss, nausea and/or vomiting; about 4-6 weeks after completing treatment for the infection
A stool sample, a breath sample, or a tissue biopsy of the stomach lining
You may need to avoid certain medications; follow any instructions you are given.
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How is the test used?
H. pylori testing is used to detect the bacteria in the digestive tract, diagnose the infection, and to evaluate whether treatment has cured the infection.
There are several different types of H. pylori testing that can be performed. The following tables summarize these tests:
Without Endoscopy
Stool/fecal antigen test Detects the presence of H. pylori antigen in a stool sample Urea breath test - A healthcare professional will take an initial sample of your breath (baseline) by having you breathe into a bag.
- You will be instructed to drink a liquid containing a substance called urea, which is specially 'labeled' and then wait about 15 minutes. During this time, if H. pylori is present in your digestive tract, the bacteria will break the urea down into labeled carbon dioxide gas, which is expelled in your breath. (Carbon dioxide is a normal part of the breath that you exhale.)
- A second breath sample is collected.
- Both the initial and second sample are sent to a lab for testing. If your second breath sample has a higher level of labeled carbon dioxide than normal, you have H. pylori in your digestive tract.
With Endoscopy: tissue biopsy sample obtained; good tests but less frequently ordered because invasive
Histology Tissue examined under a microscope by a pathologist, who will look for H. pylori bacteria and any other signs of disease that may explain a person's symptoms. Rapid urease testing H. pylori produces urease, an enzyme that allows it to survive in the acidic environment of the stomach. The laboratory test can detect urease in the tissue sample. Culture The bacteria are grown on/in a nutrient media; results can take several weeks. This test is necessary if the healthcare practitioner wants to evaluate which antibiotic will likely cure the infection. (See also Antibiotic Susceptibility Testing.) PCR (polymerase chain reaction) Fragments of H. pylori DNA are amplified and used to detect the bacteria; primarily used in a research setting. The stool antigen test and urea breath test are recommended for the diagnosis of an H. pylori infection and for the evaluation of the effectiveness of treatment. These tests are the most frequently performed because they are fast and noninvasive. (The urea breath test is not typically recommended for young children. In children, the preferred test would be the stool antigen test.) Endoscopy-related tests may also be performed to diagnose and evaluate H. pylori but are less frequently performed because they are invasive.
The testing recommendations come from guidelines by the American Gastroenterology Association (AGA), the American College of Gastroenterologists (ACG), and the Infectious Diseases Society of America (IDSA) / the American Society for Microbiology (ASM).
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When is it ordered?
Testing may be ordered when you have pain in your digestive tract and other signs and symptoms of an ulcer. Examples include:
- Abdominal pain that comes and goes over time
- Unexplained weight loss
- Indigestion
- Feeling of fullness or bloating
- Nausea
- Belching
Some people may have more serious signs and symptoms that require immediate medical attention, including sharp, sudden, persistent stomach pain, bloody or black stools, or bloody vomit or vomit that looks like coffee grounds.
H. pylori testing may also be ordered about 4 to 6 weeks after you have finished taking the prescribed antibiotics to confirm that the infection is cured. A follow-up test is not performed on every person, however.
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What does the test result mean?
A positive H. pylori stool antigen, breath test, or biopsy indicates that your signs and symptoms are likely caused by a peptic ulcer due to these bacteria. Treatment with a combination of antibiotics and other medications will be prescribed to kill the bacteria and stop the pain and the ulceration.
A negative test result means that it is unlikely that you have an H. pylori infection and your signs and symptoms may be due to another cause. However, if symptoms persist, additional testing may be done, including the more invasive tissue biopsy, to more conclusively rule out infection.
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Is there anything else I should know?
People can have digestive tract pain for many reasons; an ulcer caused by H. pylori is only one of them.
If you use antacids within the week prior to testing, the rapid urease test may be falsely negative. Antimicrobials, proton pump inhibitors, and bismuth preparations may interfere with test results.
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What is the treatment of peptic ulcer caused by H. pylori?
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Does everyone with H. pylori get ulcers?
No, many people have evidence of infection but have no symptoms of ulcerative disease. The reason why some people with H. pylori infections develop peptic ulcers and others do not is not yet understood.
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Should everyone be tested for H. pylori?
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How did I get infected with H. pylori?
The bacteria are believed to be transmitted by eating food or drinking water that has been contaminated with human stool, or possibly through contact with the stool, vomit, or saliva of an infected person. Exposure to family members with H. pylori seems to be the most likely opportunity for transmission.
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Does everyone treated for H. pylori get better?
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If I am treated and the infection goes away, can I get another H. pylori infection?
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Why is the blood test for H. pylori antibodies no longer recommended?
The American Gastroenterology Association, the American College of Gastroenterologists, the Infectious Diseases Society of America and the American Society for Microbiology do not recommend the antibody blood test for routine use in diagnosing an H. pylori infection or evaluating its treatment as the test cannot distinguish between a present and previous infection. A few healthcare practitioners still order this test, but many laboratories have stopped offering it. If performed and the blood test is negative, then it is unlikely that the person has had an H. pylori infection. If it is positive, then the presence of a current H. pylori infection should be confirmed with a stool antigen or breath test.

