Also Known As
hCG Pregnancy
Qualitative hCG
Quantitative hCG
Beta hCG
Total beta hCG
Formal Name
Human Chorionic Gonadotropin
This article was last reviewed on
This article waslast modified on March 24, 2021.
At a Glance
Why Get Tested?

To confirm a pregnancy; to help identify an ectopic pregnancy; to monitor a pregnancy that may be at risk of failing; occasionally to screen a woman for pregnancy prior to some medical treatments; as part of a panel of tests used to screen for fetal abnormalities (see First Trimester Down Syndrome Screen and Second Trimester Maternal Serum Screening)

When To Get Tested?

When you suspect that you are pregnant (testing can be done reliably by 10 days after you miss your menstrual period and some methods can detect hCG even earlier); when you have signs and symptoms that suggest you may have an ectopic pregnancy or a pregnancy that is failing; prior to some medical treatments

Sample Required?

A urine sample, preferably collected first thing in the morning, or a blood sample drawn from a vein in your arm

Test Preparation Needed?

Do not drink large amounts of fluid before collecting a urine sample for a pregnancy test because overly diluted urine may result in a false negative; no preparation is needed for a blood sample.

You may be able to find your test results on your laboratory's website or patient portal. However, you are currently at Lab Tests Online. You may have been directed here by your lab's website in order to provide you with background information about the test(s) you had performed. You will need to return to your lab's website or portal, or contact your healthcare practitioner in order to obtain your test results.

Lab Tests Online is an award-winning patient education website offering information on laboratory tests. The content on the site, which has been reviewed by laboratory scientists and other medical professionals, provides general explanations of what results might mean for each test listed on the site, such as what a high or low value might suggest to your healthcare practitioner about your health or medical condition.

The reference ranges for your tests can be found on your laboratory report. They are typically found to the right of your results.

If you do not have your lab report, consult your healthcare provider or the laboratory that performed the test(s) to obtain the reference range.

Laboratory test results are not meaningful by themselves. Their meaning comes from comparison to reference ranges. Reference ranges are the values expected for a healthy person. They are sometimes called "normal" values. By comparing your test results with reference values, you and your healthcare provider can see if any of your test results fall outside the range of expected values. Values that are outside expected ranges can provide clues to help identify possible conditions or diseases.

While accuracy of laboratory testing has significantly evolved over the past few decades, some lab-to-lab variability can occur due to differences in testing equipment, chemical reagents, and techniques. This is a reason why so few reference ranges are provided on this site. It is important to know that you must use the range supplied by the laboratory that performed your test to evaluate whether your results are "within normal limits."

For more information, please read the article Reference Ranges and What They Mean.

What is being tested?

Human chorionic gonadotropin (hCG) is a hormone produced by the placenta of a pregnant woman. Early in pregnancy, the level of hCG increases in the blood and is eliminated in the urine. A pregnancy test detects hCG in the blood or urine and confirms or rules out pregnancy.

During the early weeks of pregnancy, hCG is important in maintaining function of the corpus luteum. Production of hCG increases steadily during the first trimester (8-10 weeks) of a normal pregnancy, peaking around the 10th week after the last menstrual cycle. Levels then fall slowly during the remainder of the pregnancy. hCG is no longer detectable within a few weeks after delivery.

When a pregnancy occurs outside of the uterus (ectopic), the level of hCG in the blood increases at a slower rate. When an ectopic pregnancy is suspected, measuring the level of hCG in the blood (quantitative test) over time may be useful in helping to make a diagnosis of ectopic pregnancy.

Similarly, the hCG blood level may be abnormal when the developing baby (fetus) has a chromosome defect such as Down syndrome. An hCG test is used routinely in conjunction with a few other tests as part of screening for fetal abnormalities between 11 and 20 weeks of pregnancy. (See First Trimester Down Syndrome Screen or Second Trimester Maternal Serum Screening for more on this.)

Accordion Title
Common Questions
  • How is the test used?

    Pregnancy hCG tests that give a positive or negative result (qualitative) detect the presence of hCG. These tests may be performed by a laboratory, at a healthcare practitioner's office, or at home using a home pregnancy test kit. Methods will vary slightly but for most, a test strip is dipped into a collected cup of urine or exposed to a woman's urine stream. A colored line (or other color change) appears within the time allotted per instructions, usually about 5 minutes. For accurate test results, it is important to carefully follow the test directions. (See the article on Home Testing for more on this.) If the test is negative, it is often repeated several days later. Since hCG rises rapidly, an initial negative test can turn positive within this time period.

    Quantitative hCG testing, often called beta hCG (β-hCG), measures the amount of hCG present in the blood. It may be used to:

    • Confirm a pregnancy
    • Help diagnose an ectopic pregnancy, along with a progesterone test
    • Help diagnose and monitor a pregnancy that may be failing 
    • Monitor a woman after a miscarriage

    hCG blood measurements may also be used, along with a few other tests, as part of screening for fetal abnormalities. For more information on this use, see First Trimester Down Syndrome Screen or Second Trimester Maternal Serum Screening.

    A quantitative hCG blood test may also be used as a tumor marker in some cancers. For more on this, see the article hCG Tumor Marker.

    Occasionally, an hCG test is used to screen for pregnancy if a woman is to undergo a medical treatment, be placed on certain drugs, or have other testing, such as x-rays, that might harm the developing baby. This is usually done to help confirm that the woman is not pregnant. It has become standard practice at most institutions to screen all female patients for pregnancy using a urine or blood hCG test before a medical intervention, such as an operation, that could potentially harm a fetus.

  • When is it ordered?

    For confirming pregnancy, the timing of testing depends on how accurate a woman is about the day she expects her menstrual period as well as the method used for testing. In general, blood tests are more sensitive than urine tests and can be done two days before a woman would expect her period to start. A urine or blood hCG test can be done reliably by 10 days after a missed menstrual period. Even using a urine test, a woman may be able to determine whether she is pregnant the day she misses her period, but the result could be falsely negative. Testing may be repeated at a later date if the first test is negative but pregnancy is still suspected.

    Quantitative blood hCG tests may be ordered repeatedly over several days when a healthcare practitioner wants to identify or rule out an ectopic pregnancy or to monitor a woman after a miscarriage. In these cases, a woman may experience the normal signs and symptoms of pregnancy at first but then may develop others that indicate that the pregnancy is not progressing as expected.

    Some signs and symptoms of ectopic pregnancy include:

    • Abnormal vaginal bleeding—because a woman is pregnant, she may not have a regular period but then may have light bleeding or spotting with an ectopic pregnancy
    • Low back pain
    • Pain or cramping in the lower abdomen or on one side of the pelvis

    If untreated, signs and symptoms may get worse and may include:

    • Dizziness, weakness
    • Feeling faint or fainting
    • Low blood pressure
    • Pain in the shoulder area
    • Sudden, sharp pain in the pelvic area
    • Fever, flu-like symptoms
    • Vomiting

    The area around an ectopic pregnancy may rupture and start to bleed, and, if undiagnosed, can lead to cardiac arrest and death.

    An hCG test may be ordered prior to a medical procedure or treatment that might be harmful during pregnancy.

  • What does the test result mean?

    A negative hCG result means that it is unlikely that a woman is pregnant. However, tests performed too early in a pregnancy, before there is a significant hCG level, may give false-negative results. The test may be repeated in one week if there is a strong possibility of pregnancy.

    A positive hCG means that a woman is likely pregnant. However, false-positive tests can occur if a woman is post-menopausal or taking hormone supplements.

    The blood level of hcG in a woman with an ectopic pregnancy usually rises at a slower rate than normal. Typically, hCG levels double every 48 to 72 hours for the first four weeks of a normal pregnancy, then slow to every 96 hours by six weeks. Those with failing pregnancies will also frequently have a longer doubling time early on or may even show falling hCG concentrations during the doubling period. hCG concentrations will drop rapidly following a miscarriage. If hCG does not fall to undetectable levels, it may indicate remaining hCG-producing tissue that will need to be removed (dilation and curettage – D&C).

    A high hCG level may indicate that the pregnancy dating has been miscalculated, that there is a molar pregnancy, or that it is a multiple pregnancy.

  • What can cause a false-negative urine hCG?

    Urine hCG tests may give a false-negative result if the urine is too diluted or if testing is done too soon in the pregnancy. Certain drugs such as diuretics and promethazine (an antihistamine) may cause false-negative urine results.

  • What can cause a false-positive result?

    Drugs such as antihistamines, anti-anxiety medications, diuretics, anti-convulsants, anti-parkinson drugs, hypnotics, and tranquilizers may cause false-positive results.

    The presence of protein in the urine (proteinuria), blood in the urine (hematuria), or excess pituitary gonadotropin may also cause a false-positive urine hCG test.

    There are reports of false-positive blood hCG results due to the presence of certain types of antibodies that some individuals produce or fragments of the hCG molecule. Generally, if results are questionable, they may be confirmed by testing with a different method.

  • How does the test that I do at home myself compare with the results of a test done in a lab?

    Home pregnancy testing is very similar to qualitative urine hCG testing performed in the laboratory, but there are factors surrounding its use that are important to note.

    • Home tests come with very specific directions that must be followed explicitly. If you are using a home test, follow the directions extremely carefully (see Home Testing). There can be variability in sensitivity to detecting the presence of hCG with different brands of home pregnancy kits.
    • Home tests are sometimes done too soon after the missed menstrual cycle to result in a positive test. It typically takes 10 days after a missed menstrual period before the presence of hCG can be detected by the urine test.
    • All urine hCG tests should be done on a first morning urine sample, if possible. Urine becomes more dilute after ingestion of liquids (coffee, juice, water, etc.) and urine hCG concentrations may become too low to register as positive.

    Generally, when used correctly, the home test should produce the same result as the urine hCG test done by your healthcare practitioner. Blood testing for hCG is more sensitive than urine hCG testing, so sometimes a blood test will indicate pregnancy when the urine test is negative.

  • When is a blood hCG test ordered instead of a urine hCG?

    Since hCG is not normally detected in the urine of a non-pregnant woman, a urine hCG is enough to confirm a pregnancy. This can also be done with a qualitative blood hCG test. Sometimes, however, it is important to know how much hCG is present to evaluate a suspected ectopic pregnancy or to monitor a woman following a miscarriage. In these circumstances, a healthcare practitioner will order a quantitative blood hCG test.

  • How many days after a miscarriage would it take for a urine pregnancy test to show a negative result?

    Urine hCG decreases at about the same rate as serum hCG, which can take anywhere from 9 to 35 days, with a median of 19 days. However, the timeframe for when an hCG result will be negative is dependent on what the hCG level was at the time of the miscarriage. Frequently, miscarriages are monitored with quantitative blood hCG testing. If the levels of hCG do not fall to undetectable levels, some hCG-producing tissue may remain and have to be removed.

  • What is an ectopic pregnancy?

    An ectopic pregnancy occurs when the fertilized egg (ovum) implants somewhere other than in the uterus. Nearly all ectopic pregnancies occur in the fallopian tube, giving the condition the moniker of "tubal pregnancy." This is a serious condition needing immediate treatment. Women with ectopic pregnancies often have sharp, stabbing abdominal or pelvic pain and uterine bleeding. Usually, abnormally low levels of hCG are produced in ectopic pregnancies with slower-than-normal rates of increase. A physician that suspects an ectopic pregnancy might also test progesterone levels, which will be lower than in a non-ectopic pregnancy.

    In the case of an ectopic pregnancy, hCG levels need to be rechecked regularly until they are undetectable. If hCG levels remain high, it might mean that the ectopic tissue wasn't removed, which could require treatment by surgery or methotrexate, which causes the body to absorb the pregnancy tissue.

Health Professionals – LOINC

Logo for LOINC from RegenstriefLOINC Observation Identifiers Names and Codes (LOINC®) is the international standard for identifying health measurements, observations, and documents. It provides a common language to unambiguously identify things you can measure or observe that enables the exchange and aggregation of clinical results for care delivery, outcomes management, and research. Learn More.

Listed in the table below are the LOINC with links to the LOINC detail pages. Please note when you click on the hyperlinked code, you are leaving Lab Tests Online and accessing

LOINC LOINC Display Name
21198-7 HCG.beta subunit Qn
20415-6 HCG.beta subunit IA 3rd IS Qn
55869-2 HCG.beta subunit [Mass/Vol]
2110-5 Beta HCG (pregnancy test) Ql
2112-1 Beta HCG (pregnancy test) Ql (U)
2111-3 HCG.beta subunit [Moles/Vol]
2114-7 HCG.beta subunit (U) [Moles/Vol]
19080-1 HCG Qn
83086-9 HCG IA Qn
25372-4 HCG Qn (U)
20994-0 HCG [Interp]
34670-0 HCG [Mass/Vol]
2118-8 HCG (pregnancy test) Ql
80385-8 HCG (pregnancy test) IA.rapid Ql (S)
2106-3 HCG (pregnancy test) Ql (U)
80384-1 HCG (pregnancy test) IA.rapid Ql (U)
2119-6 HCG [Moles/Vol]
2107-1 HCG (U) [Moles/Vol]
View Sources

Sources Used in Current Review

Ectopic Pregnancy. American Pregnancy Association. Available online at Accessed on 10/17/18.

(May 12, 2015) Reference ranges and determinants of total hCG levels during pregnancy: The General R Study. European Journal of Epidemiology. Available online at Accessed on 10/17/18.

(October 4, 2016) HCG Blood Test — Qualitative. MedlinePlus. Available online at Accessed on 10/18/18.

(November 21, 2017) Human Chorionic Gonadotropin (HCG). NorthShore University Health System Health Encyclopedia. Available online at Accessed on 10/18/18.

(October 22, 2018) Human Chorionic Gonadotropin (HCG): The Pregnancy Hormone. American Pregnancy Association. Available online at Accessed on 10/18/18.

(May 21, 2018) What is a False-Positive Pregnancy Test? Ohio Health. Available online at Accessed on 10/18/18.

Sources Used in Previous Reviews

Thomas, Clayton L., Editor (1997). Taber's Cyclopedic Medical Dictionary. F.A. Davis Company, Philadelphia, PA [18th Edition].

Pagana, Kathleen D. & Pagana, Timothy J. (2001). Mosby's Diagnostic and Laboratory Test Reference 5th Edition: Mosby, Inc., Saint Louis, MO.

(2003 June 23). Free Beta-Subunit of Human Chorionic Gonadotropin (Free b-hCG) Enzyme Immunoassay Test Kit. BioCheck, Inc. [On-line package insert]. PDF available for download at

Newberger, D. (2000 August 15). Down Syndrome: Prenatal Risk Assessment and Diagnosis. American Family Physician [On-line journal]. Available online at

Qin, Q., et. al. (2002). Point-of-Care Time-resolved Immunofluorometric Assay for Human Pregnancy-associated Plasma Protein A: Use in First-Trimester Screening for Down Syndrome [Pages 1-3 of 20]. Clinical Chemistry 48:473-483 [On-line journal]. Available online at

(2000). Down Syndrome Screening. NTD Laboratories, Inc. [On-line information]. Available online at

Hoffman, B. and Johnson, J. (2004). Toronto's Mount Sinai Hospital offers First-Trimester Screening Across Ontario. Healthcare Quarterly 7(2). PDF available for download at

Paralloi, A. (August 13, 2003, Edited) Second Trimester Maternal Serum Screening Programmes for the Detection of Down's Syndrome. Geneva Foundation for Medical Education and Research [On-line information, 8th Post Graduate Course]. Available online through

First Trimester Prenatal Screening. Alfigen, The Genetics Institute, Laboratory Services [On-line information]. Available online at

(1999 March 18). Prenatal Program Pushes the Frontiers of Diagnosis. Cedars-Sinai Medical Center [On-line News release]. Available online at

Free beta hCG [I-125] IRMA kit (RK-820CT) - coated tube. IZOTOP [On-line test information]. Available online at

Beta-hCG, Serum Quantitative=. ARUP's Guide to Clinical laboratory Testing [On-line information]. Available online at

Spencer, K. What is Free Beta hCG. Down Syndrome Screening Research [On-line information]. Available online at

Ultra-Screen First Trimester Prenatal Screening. Center for Medical Genetics [On-line brochure]. PDF available for download at

Vankrieken, L., et. al. (2000). HCG and Subunits: DPC Assay Specificities and Clinical Utility in Obstetrical Care and Oncology. Immulite [DPC Technical Report]. PDF available for download through

Framarin, A. (2003) First-trimester prenatal screening for Down syndrome and other aneuploidies. Agence d’e’valuation des technologies et des modes d’intervention en sante’ [On-line report, Quebec]. PDF available for download through

(2000). What is Ultra-Screen. NTD Laboratories, Inc. [On-line information]. Available online at

Shaskan, J. (2004 May 31). Amniocentesis and CVS Tests Decline Despite Increase in Number of Older Mothers. ACOG [On-line news release]. Available online at

Wu, A. (2006). Tietz Clinical Guide to Laboratory Tests, Fourth Edition. Saunders Elsevier, St. Louis, Missouri. Pp 252-255.

Vorvick, L. (Updated 2010 November 21) HCG blood test – qualitative. MedlinePlus Medical Encyclopedia [On-line information]. Available online at Accessed March 2011.

Gaufberg, S. (Updated 2010 April 16) Early Pregnancy Loss. eMedicine [On-line information]. Available online at Accessed March 2011.

Pagana, K. D. & Pagana, T. J. (© 2011). Mosby's Diagnostic and Laboratory Test Reference 10th Edition: Mosby, Inc., Saint Louis, MO. Pp 558-560.

Grenache, D. et. al. (Updated 2011 January) hCG Testing – hCG. ARUP Consult [On-line information]. Available online at Accessed March 2011.

(© 1995–2011). Unit Code 80678: Human Chorionic Gonadotropin (hCG), Quantitative, Pregnancy, Serum Mayo Clinic, Mayo Medical Laboratories [On-line information]. Available online at Accessed March 2011.

Ann M. Gronowski, Corinne R. Fantz, Curtis A. Parvin, Lori J. Sokoll, Carmen L. Wiley, Mark H. Wener, and David G. Grenache. Use of Serum FSH to Identify Perimenopausal Women with Pituitary hCG. Clin. Chem., Apr 2008; 54: 652 - 656.

One-Year Experience with Day-of-Surgery Pregnancy Testing Before Elective Orthopedic Procedures. Kahn, RL et al. Anesthesia & Analgesia April 2008. Vol. 106. No. 4 Pp 1127-1131.

Farinde, A. (Updated 2014 July 2). Human Chorionic Gonadotropin (hCG). Medscape Drugs & Diseases [On-line information]. Available online at Accessed September 2014.

(Updated 2014 June 5). Pregnancy. U.S. Food and Drug Administration Medical Devices [On-line information]. Available online at Accessed September 2014.

Grenache, D. and Lehman, C. (Updated 2012 November). hCG Testing – hCG. ARUP Consult [On-line information]. Available online at Accessed September 2014.

Rollins, G. (2012 March 8). Taking a New Look at the hCG Discriminatory Level. Clinical Laboratory Strategies [On-line information]. Available online at Accessed September 2014.

Grenache, D. (2013). Identifying the Pregnant Patient, There's More to Know than "Yes" or "No." AACC Presentation [On-line information]. Available online at Accessed September 2014.

Grenache, D. (2011). Human Chorionic Gonadotropin. Clinical Chemistry Trainee Council Pearls of Laboratory Medicine [On-line information]. Available online at Accessed September 2014.

Pagana, K. D. & Pagana, T. J. (© 2011). Mosby's Diagnostic and Laboratory Test Reference 10th Edition: Mosby, Inc., Saint Louis, MO. Pp 558-560.

Manual of Laboratory and Diagnostic Tests, 8th ed. (2009) Lippincott Williams & Wilkins: Philadelphia.

Sepilian V (September 2, 2014). Ectopic Pregnancy. Medscape Reference. Available online at Accessed September 2014.

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